Port Adelaide midfielder Jason Horne-Francis has been ruled out of Sunday's clash against Richmond at the MCG.
Horne-Francis trained with the group on Friday but has pulled up with a hamstring issue.
No clear timeline for his return has been confirmed.
Reports have indicated that the former number 1 Pick suffered a minor strain and is likely to miss more than just Sunday's game.
With teams yet to be finalised, defender Tom Clurey comes into the extended squad of 26 to replace Horne-Francis.
Forward Mitch Georgiades is also in the frame for selection after coming back from an ACL injury almost 11 months ago.
Georgiades played in the SANFL last weekend as he continues to push his case for senior selection.
Jed McEntee's position is up for grabs, with the small forward entering concussion protocols following the win against West Coast in Round 1.
The club will celebrate Travis Boak's 350th game when they face the Tigers on Sunday.
Final teams are to be announced at 5 pm (AEST).